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KMID : 0380020110260050400
Korean Journal of Biotechnology and Bioengineering
2011 Volume.26 No. 5 p.400 ~ p.406
Enhancement of Saccharification Yield of Ulva pertusa Kjellman by High Pressure Homogenization Process for Bioethanol Production
Choi Woon-Yong

Lee Choon-Geun
Ahn Ju-Hee
Seo Yong-Chang
Lee Sang-Eun
Jung Kyung-Hwan
Kang Do-Hyung
Cho Jeong-Sub
Choi Geun-Pyo
Lee Hyeon-Yong
Abstract
This study was investigated to improve the saccharification yield of Ulva pertusa Kjellman by the high pressure homogenization process. It was found that the high pressure homogenization pretreatment effectively destructed the cell wall structures only by using water. The high pressure homogenization process was operated under various conditions such as 10000, 20000 or 30000 psi with different recycling numbers. The optimal condition was determined as 30000 psi and 2 pass of recycling numbers and the sugar conversion yields were 16.02 (%, w/w) of glucose and 14.70 (%,w/w) of xylose, respectively. In the case of enzymatic treating the hydrolyzates with 5 FPU/glucan of celullase and 100 units/mL of amyloglucosidase, 65.8% of carbohydrates was converted into glucose. Using the hydrolysates of Ulva pertusa Kjellman, 48.7% of ethanol was obtained in the culture S.cerevisiae. These results showed that the high pressure homogenization process could efficiently hydrolyze the marine resource by using only water for bioethanol production.
KEYWORD
Ulva pertusa kjelmann, high pressure homogenization process, sugar yields, HMF, bioethanol production
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