KMID : 0380020110260050400
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Korean Journal of Biotechnology and Bioengineering 2011 Volume.26 No. 5 p.400 ~ p.406
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Enhancement of Saccharification Yield of Ulva pertusa Kjellman by High Pressure Homogenization Process for Bioethanol Production
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Choi Woon-Yong
Lee Choon-Geun Ahn Ju-Hee Seo Yong-Chang Lee Sang-Eun Jung Kyung-Hwan Kang Do-Hyung Cho Jeong-Sub Choi Geun-Pyo Lee Hyeon-Yong
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Abstract
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This study was investigated to improve the saccharification yield of Ulva pertusa Kjellman by the high pressure homogenization process. It was found that the high pressure homogenization pretreatment effectively destructed the cell wall structures only by using water. The high pressure homogenization process was operated under various conditions such as 10000, 20000 or 30000 psi with different recycling numbers. The optimal condition was determined as 30000 psi and 2 pass of recycling numbers and the sugar conversion yields were 16.02 (%, w/w) of glucose and 14.70 (%,w/w) of xylose, respectively. In the case of enzymatic treating the hydrolyzates with 5 FPU/glucan of celullase and 100 units/mL of amyloglucosidase, 65.8% of carbohydrates was converted into glucose. Using the hydrolysates of Ulva pertusa Kjellman, 48.7% of ethanol was obtained in the culture S.cerevisiae. These results showed that the high pressure homogenization process could efficiently hydrolyze the marine resource by using only water for bioethanol production.
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KEYWORD
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Ulva pertusa kjelmann, high pressure homogenization process, sugar yields, HMF, bioethanol production
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